DNA

Part:BBa_K2100078:Design

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-19)


pEXPR EGSH - 4kturn: mKate


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1265
    Illegal XbaI site found at 2225
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1265
    Illegal NheI site found at 810
    Illegal NheI site found at 1076
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
    Illegal NotI site found at 2239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1265
    Illegal BglII site found at 1388
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1265
    Illegal XbaI site found at 2225
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1265
    Illegal XbaI site found at 2225
    Illegal PstI site found at 1758
    Illegal PstI site found at 2232
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4096
    Illegal SapI.rc site found at 687
    Illegal SapI.rc site found at 3478


Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

We synthesized this part using LR Gateway cloning.

References