DNA
Part:BBa_K2100078:Design
Designed by: Colleen Foley Group: iGEM16_MIT (2016-10-19)
pEXPR EGSH - 4kturn: mKate
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1265
Illegal XbaI site found at 2225
Illegal PstI site found at 1758
Illegal PstI site found at 2232 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1265
Illegal NheI site found at 810
Illegal NheI site found at 1076
Illegal PstI site found at 1758
Illegal PstI site found at 2232
Illegal NotI site found at 2239 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1265
Illegal BglII site found at 1388 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1265
Illegal XbaI site found at 2225
Illegal PstI site found at 1758
Illegal PstI site found at 2232 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1265
Illegal XbaI site found at 2225
Illegal PstI site found at 1758
Illegal PstI site found at 2232 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4096
Illegal SapI.rc site found at 687
Illegal SapI.rc site found at 3478
Design Notes
This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
We synthesized this part using LR Gateway cloning.